Synseed Production for Storage and Conservation of Ochradenus Baccatus Delile

Ochradenus baccatus is a medicinal plant of high value, spread over sandy and stony places of Kingdom of Saudi Arabia and most of the desert regions of Egypt. This species contains several flavanoids and specific constituents which are important as these have already been effectively used in lowering cholesterol in the blood of rats and high inhibition potential of the malarial parasite (Plasmodium falciparum). Synseeds were produced from stem segments and apical bud of O. baccatus growing In vitro. Two sets of synseeds were produced, one non-dried and the other dried under running laminar air-flow for 30 min. Regeneration and regrowth were evaluated for 16 weeks storage under various temperatures (4, 8 and 12oC). The maximum frequency of conversion into plantlets was achieved on the MS medium containing 1.0 μM BA in encapsulated nodal segments stored at 4oC. Rooting in these shoots was induced by the pulse treatment of 100 μM IBA for 10 days, and the rooted shoots were transferred on the MS medium devoid of any PGR. Fair percent rooting occurred after one week of transfer on the MS medium. Plantlets were successfully established. No phenotypic variations were observed between the synseed originated plants with mother plant. Genetic stability of synseed grown plants and mother plant was evaluated by inter-simple sequence repeat (ISSR) marker. The mother plant as well as regenerated plants from synseed resulted in a monomorphic banding pattern developed from ISSR markers confirming genetic stability among the clones. This protocol will help multiply and conserve the plant as well as for short-term storage of germplasm for commercial use and exchange.